A. Anesthesia containing 3% sevoflurane administered for 2 hours daily for three days in P6 mice increased their levels of LC3-II (lanes 3 to 4) compared to those in the control condition (lanes 1 to 2). 3-MA treatment alone (lanes 5 to 6) did not alter the levels of LC3-II as compared to those in the control condition, but the 3-MA treatment (lanes 7 to 8) attenuated the sevoflurane-induced increase in the LC3-II levels of mice compared those receiving the anesthetic sevoflurane (lanes 3 to 4). There was no significant difference in the beta-Actin levels among these treatments. B. Quantification of the Western blot showed that the anesthetic sevoflurane increased the LC3-II levels (gray bar versus white bar) and that 3-MA attenuated the sevoflurane-induced increase in the LC3-II levels (net bar versus gray bar) (F = 21.96, P < 0.0001, two-way ANOVA). C. The quantification of the Western blots showed that the anesthetic sevoflurane increased the ratio of LC3-II/LC3-I in mice compared to those in the control condition (white bar versus black bar), and 3-MA attenuated the sevoflurane-induced increase in the ratio of LC3-II/LC3-I (net bar versus gray bar) (F = 6.262, P = 0.0211, two-way ANOVA). LC = microtubule-associated protein 1 light chain; 3-MA = 3-Methyladenine; ANOVA = analysis of variance; NS = normal saline.