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. 2019 May 20;14(5):e0216372. doi: 10.1371/journal.pone.0216372

Fig 2. Sevoflurane decreased p62 level in the hippocampus of young mice.

Fig 2

A. The anesthetic sevoflurane decreased the levels of p62 (lanes 3 to 4) in mice compared to those in the control condition (lanes 1 to 2). The 3-MA treatment alone (lanes 5 to 6) did not significantly alter the p62 levels in mice to those receiving saline treatment, whereas the pretreatment of 3-MA (lanes 7 to 8) attenuated the sevoflurane-induced decrease in the p62 levels. There was no significant difference in the beta-Actin levels among these treatments. B. The quantification of the Western blot showed that the anesthetic sevoflurane attenuated the p62 levels (gray bar versus white bar), and 3-MA attenuated the sevoflurane-induced reduction in the levels of p62 (net bar versus gray bar) (F = 4.494, P < 0.0467, two-way ANOVA). p62 = Sequestosome 1(SQSTM1); 3-MA = 3-Methyladenine; ANOVA = analysis of variance.