Table 2. Primer sequences for the qPCR of rumen microorganisms.

Item	Forward/reverse (F/R) primer sequences (5’-3’)	Amplification size/bp	GenBank number
Fungi	F: TGACTCAACACGGGGAAACT R: CCAACTAAGAACGGCCATGC	105	JX240418.1
Protozoa	F: TGACTCAACACGGGGAAACT R: TCCACCAACTAAGAACGGCC	109	AJ810076.1
Bacteria	F: CCTACGGGAGGCAGCAG R: ATTACCGCGGCTGCTGG	181	[18]
R. flavefaciens†	F: TCT GGA AAC GGA TGG TA R:CCTTTAAGACAGGAGTTTACAA	259	[19]
R. ablus‡	F: ATGCCGCGGTGAATACGTT R: TTCGACTGCTTCCTCCTTGC	107	X85098.1
F. succinogenes§	F: GATGAGCTTGCGTCCGATT R: ATTCCCTACTGCTGCCTCC	139	EU606019.1

^†R. flavefaciens = Ruminococcus flavefaciens

^‡R. albus = Ruminococcus albus

^§F. succinogenes = Fibrobacter succinogenes