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. 2019 May 20;14(5):e0217002. doi: 10.1371/journal.pone.0217002

Fig 4. DLL1 downregulation promotes apoptosis of MCF-7 cells.

Fig 4

(A-C) MCF-7, BT474 and MDA-MB-231 cells were not-transfected (NT) or transfected with DLL1-siRNAs (DLL1-si1/2), or negative control (Ctr) siRNA. At 92 hours (MCF-7) and 120 hours (BT474 and MDA-MB-231) after transfection cells were collected, stained with annexin-V and apoptosis analyzed by flow cytometry. Representative dot plots showing the percentage of apoptosis at each condition are represented. Graph shows percentage of apoptosis (mean + SD) of at least three independent assays, each one performed in triplicate. *, P < 0.05, compared with cells transfected with Ctr-siRNA.