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. 2019 May 20;14(5):e0217002. doi: 10.1371/journal.pone.0217002

Fig 5. DLL1 downregulation in MCF-7 cells upregulate genes involved in cell cycle arrest and represses genes that promote cell cycle progression, proliferation and survival.

Fig 5

Cells were transfected with DLL1-siRNA1 or Ctr-siRNA. (A) At 55–70 hours following transfection total RNA was extracted and the expression of genes that control cell cycle arrest (1), cell cycle transition (2), proliferation (3), and survival (4) were quantified by qRT-PCR. The values were normalized against the HPRT1 mRNA levels in the same sample and calculated as fold change relative to control cells transfected with Ctr-siRNA. Results are represented as mean values (+ SD) from four independent assays. (B) The expression levels of the indicated proteins were determined by immunoblotting 72 hours after transfection in total soluble protein extracts. α-tubulin was used as an internal control. The graph shows relative expression of these proteins in cells transfected with DLL1-siRNA1 as compared to Ctr-siRNA transfected cells of at least three independent experiments. *, P < 0.05, compared with cells transfected with Ctr-siRNA.