Fig 3. Hydrogen peroxide–induced mutagenesis is not caused by increased rates of incorporation errors by DNA polymerases delta and epsilon.

A. Frequency of spontaneous and peroxide-induced CanR mutations and CanR Red closely spaced mutations in ssDNA triple reporter in parental wild-type and derivate pol3-5DV strains had been measured simultaneously for four (wt) and 24 (pol3-5DV) independent cultures, as described in Materials and methods. P values were determined by the Mann–Whitney test. See also S1 Data. B. Frequency of spontaneous and peroxide-induced CanR mutations and CanR Red closely spaced mutations in ssDNA triple reporter in parental wild-type and derivate pol2-4 strains had been measured simultaneously for eight (wt) and eight (pol2-4) independent cultures, as described in Materials and methods. P values were determined by the Mann–Whitney test. See also S1 Data. CanR Red, canavanine-resistant red; ssDNA, single-strand DNA; wt, wild-type.