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. 2019 May 8;13(5):e0007354. doi: 10.1371/journal.pntd.0007354

Fig 4. Monocyte expression of TLR4 is not altered by carriage of TLR5:c.1174C>T.

Fig 4

Human monocytes isolated from whole blood were left unstimulated or stimulated with E. coli LPS 10 ng/ml for four hours. RNA was isolated from cell lysates and TLR4 expression was determined by quantitative RT-PCR and is expressed relative to GAPDH. TLR5:c.1174C>T genotype (CC, CT, or TT) was determined for each individual by ABI Taqman assay on matched saliva DNA. n = 4 (CC), n = 3 (CT+TT). Mean values ± standard deviation are displayed. P>0.05 for the comparison of relative TLR4 expression between CC and CT+TT subjects for both conditions using the t test.