Skip to main content
. 2019 Apr 6;18(8):824–833. doi: 10.1080/15384101.2019.1593648

Figure 6.

Figure 6.

TSC1 negatively regulates primordial follicle assembly in the mouse ovary. Real-time PCR (a) and western blot (b) analyses of TSC1 in 0.5dpp ovaries transfected with TSC1 siRNA or respective controls and cultured for 4 days. (c, d) The 0.5dpp ovaries were transfected with TSC1 NC or TSC1 siRNA and then collected 4 days later to count the germ cells in cyst (dashed red circle) and primordial follicles (black circle). The representative images were shown in (c), and percentage of germ cells in cysts, primordial follicles was shown in (d). (e) Real-time PCR analysis of growth-related genes in TSC1 NC or TSC1 siRNA treated ovaries. The levels of mRNA expression in TSC1 siRNA treated group were normalized to the control groups. * p < 0.05.