Figure 1.
Fluorescently labeled protein tags to monitor protein degradation. (a): A protein of interest is fused to a SNAP/Halo/Clip-tag, which can be pulse-labeled with a fluorescent ligand. Upon washing of the residual dye, the decay of the labeled protein population can be observed. (b): Protein degradation follows an exponential decay. A0: Initial fluorescence intensity, b: decay rate, T1/2: half-life. (c): Schematic representation of how SNAP/Halo/Clip-tags can be used to identify changes in protein degradation.