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. 2019 Apr 24;18(9):990–1000. doi: 10.1080/15384101.2019.1608127

Figure 3.

Figure 3.

Effect of miR-1225 expression on the proliferation of PANC-1 and AsPC-1 cells. (a, b) Expression of miR-1225 in PANC-1 and AsPC-1 cells transfected with the miR-1225 inhibitor (Inhibitor) or NC inhibitor (NC), as measured by qPCR. (c, d) Proliferation rate of PANC-1 and AsPC-1 cells was measured at 24, 48, and 72 h post-transfection using the EdU assay. (e, f) Soft agar colony formation assay of the PANC-1 and AsPC-1 cells transfected with the miR-1225 inhibitor or NC inhibitor. The lower panel indicates the number of colonies formed in each group. Data are represented in the form of mean ± SD. *P < 0.05, **P < 0.01, ***P< 0.001.