Figure 5.

Sequential LTX-401 treatment with double immune checkpoint blockade exhibits systemic antitumor immunity C57BL/6 mice were inoculated with murine fibrosarcoma MCA205 subcutaneously on one side and four days later on the contralateral flank. Palpable primary tumors arising from the first injection were treated with sequential injections of 0.25 mg LTX-401 as indicated in (A). Immune checkpoint blockade was mounted by sequential injections of double immune checkpoint blockade with monoclonal antibodies targeting CTLA-4 or PD-1 at day 6, 9 and 12 post treatment. Immune checkpoint inhibition alone did not control tumor growth of LTX-401-treated primary (B) and distant (C) tumors. In contrast, combination of sequential LTX-401 treatment with double immune checkpoint blockade cleared 100% of LTX-401-treated primary tumors (D) and depicted abscopal effects on distant tumors (E).