Fig. 1. CD11c-cre-Irf4^f/f mice harbor higher numbers of antigen-specific CD4⁺ and CD8⁺ IFNγ⁺ T cells in the mLN after IAV infection.

WT and KO mice were infected i.n. with a sublethal dose of PR8 IAV. (a) Weight loss and recovery of WT and KO mice (n=3 each) over 12 days p.i. with PR8 virus. Shown are the mean and SEM. Differences in WT and KO mice were not significant as determined by a multiple t test with the Bonferroni-Sidak method, (b) Total numbers of mLN cells on day 12 p.i. (c) Total numbers of CD45⁺ cells in the lung on day 12 p.i. (d) Numbers of CD8⁺ T cells binding H-2D^b/NP366-374 tetramers (Tet) and (e) numbers of CD4⁺ T cells binding I-A^b/NP311-325 tetramers in the mLN on days 8, 10 and 12 p.i. (f) Numbers of CD8⁺ T cells producing IFNγ after incubation of mLN cells (day 12 p.i.) in vitro with or without NP366-374 peptide. (g) Numbers of CD4⁺ T cells producing IFNγ after incubation of mLN cells (day 12 p.i.) in vitro with or without NP311-325 peptide. Gating of T cells is shown in Fig. S2a. The data are compiled from 1-2 (a-e) or 4 (f-g) independent experiments, each with 3-4 animals per WT or KO group. Symbols represent individual mice, with the mean and SD indicated. Significance (b-g) was evaluated using Mann-Whitney tests, with p values indicated.