Fig. 2. CD11c⁺ lung DCs in CD11c-cre-Irf4^f/f mice show decreased Il10 and increased Il12a RNA upon IAV infection.

(a) Expression of IRF4 determined by intracellular staining of the indicated DC subsets in lung and mLN of WT and KO mice on day 5 p.i. Gating of DC subsets is shown in Fig. S2b. (b) Relative levels of Il10 and Il12a RNA in total CD11c⁺ lung cells on days 0 and 3 p.i. determined by qPCR. (c) Levels of Il10, Il12a and Il12b RNA relative to Gapdh RNA in sorted lung DC subsets on day 5 p.i. determined by qPCR and compiled from 2 independent experiments. In all panels, symbols represent individual mice, with the mean and SD indicated. ND, not determined due to absence of the CD11b⁺P1 subset in KO mice. Significance was evaluated using a t test (panel b) or one-way ANOVA with a Tukey’s multiple comparison test (panels a-c), with p values indicated.