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miR-125-5p directly regulated IRF1. (a) Software prediction (microRNA.org) showed that miR-125a-5p might combine with IRF1 3 ‘UTR. (b) Double luciferase reporter assay was used to determine whether miR-125-5p directly regulated IRF1 in VSMCs. (c) Overexpression of miR-125a-5p down-regulated IRF1 expression, while the interference of miR-125a-5p up-regulated IRF1 expression. *P < 0.05, vs NC or per-NC.
