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. 2019 Mar 24;13(1):169–181. doi: 10.1080/19336918.2019.1594497

Figure 4.

Figure 4.

The expression of ARHGEF4 and ARHGEF6 was significantly upregulated in HSCs cultured in 3D FCM. (a). The significantly upregulated expression of ARHGEF4 mRNA in HSCs cultured in 3D FCM was confirmed by real-time PCR. LX-2 cells were cultured on 2D PS or in 3D FCM, then total RNA was isolated, and real-time PCR was performed to detect ARHGEF4 mRNA expression. *p < 0.05, data are the means ± SD. (b). The upregulated expression of ARHGEF6 mRNA in HSCs cultured in 3D FCM was confirmed by real-time PCR. LX-2 cells were cultured on 2D PS or in 3D FCM, then total RNA was isolated, and real-time PCR was performed to detect ARHGEF6 mRNA expression. *p < 0.05, data are the means ± SD. (c). The upregulated expression of Asef (ARHGEF4) protein and αPix (ARHGEF6) protein were confirmed by Western blotting. LX-2 cells were cultured on 2D PS or in 3D FCM for 1 d, and the proteins were obtained by treatment with lysis buffer or collagenase before lysis buffer for cells cultured in 3D FCM. Western blotting was performed to detect the protein expression of Asef and αPix.