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The mRNA expression of podosome-related genes after ARHGEF4 or ARHGEF6 knockdown in LX-2 cells cultured in 3D FCM. LX-2 cells were treated with ARHGEF4 siRNA or ARHGEF6 siRNA for 2 d and cultured in 3D FCM for another day. Total RNA was then isolated, and real-time PCR was performed to detect the mRNA expression of podosome-related genes. *p < 0.05, data are the means ± SD.
