Figure 3.

The cell adhesion ability of T47D cells on different ECM proteins was determined. (a) Using different concentrations (0.1, 1, and 10 μg/ml) of three types (fibronectin, type I, and type IV collagens) of ECM proteins, cell adhesion ability between cell dissociation buffer- and trypsin-EDTA (1X; 21 μM)-detached T47D cells was assessed. The BSA group was the negative control. Finally, we chose a 1 μg/ml dose of ECM proteins for the following experiments. (b) (c) Different concentrations (0, 1, 10, and 100 μM) of nicotine and NNK (0, 10, 100, and 1000 nM) were used to determine the influence the adhesion ability of dissociation buffer- and trypsin-detached T47D cells cultured on three types of ECM proteins (1 μg/ml). P-values were determined with Student’s t-test; *P < 0.05 and **P < 0.01. All experiments were repeated more than 3 times (n > 3).