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. 2019 Jan 25;98(6):2380–2391. doi: 10.3382/ps/pez017

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© The Author 2019. Published by Oxford University Press on behalf of Poultry Science Association.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com.

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Figure 1. — Grouping by PCA of adult hens (A), hatchlings (B), yolk (C), albumen (D), and day 8 chicks (E) on the basis of IgM and IgG antibody titers to 5 antigens: KLH, PC-BSA, PC-OVA, dsDNA, and OVA. Diagrams include 15 birds or specimens from each line (enveloped) that were analyzed with ELISA. ‘Eigen values’ explaining the amount of variation were 60% horizontal and 20% vertical (A); 64% horizontal and 13% vertical (B); 58% horizontal and 17% vertical (C); 74% horizontal and 19% vertical (D); 73% horizontal and 12% vertical (E). Diagrams include antigen and isotype combinations (arrows). Length and direction of arrows indicate the contribution of the antigen–isotype combination to the grouping of the lines. Small fonts after the antigen label represent the isotype m: IgM and g: IgG.