Appendix 1.

Coating concentrations of antigens and conjugate dilutions used.

		Antigen Coating	Serum Starting	Dilution		Conjugate
Antigen	Sample	Concentration (μg/mL)	Dilution	Steps	Conjugate	Dilution
PC-BSA	Hen Plasma	1	40	4	IgM	10,000
			40	4	IgG	10,000
	Yolk	2	10	2	IgM	10,000
			10	2	IgG	10,000
	Albumen	2	10	2	IgM	10,000
			10	2	IgG	10,000
	Hatchling Plasma	1	40	4	IgM	10,000
			40	4	IgG	10,000
	Day 8 Plasma	1	40	4	IgM	5,000
			40	4	IgG	20,000
KLH	Hen Plasma	1	40	4	IgM	10,000
			40	4	IgG	10,000
	Yolk	1	40	4	IgM	10,000
			40	4	IgG	10,000
	Albumen	1	10	2	IgM	10,000
			10	2	IgG	10,000
	Hatchling Plasma	1	40	4	IgM	10,000
			40	4	IgG	10,000
	Day 8 Plasma	1	40	4	IgM	5,000
			40	4	IgG	10,000
dsDNA	Hen Plasma	0.5	40	4	IgM	10,000
			40	4	IgG	10,000
	Yolk	2	10	2	IgM	5,000
			10	2	IgG	5,000
	Albumen	2	10	2	IgM	5,000
			10	2	IgG	5,000
	Hatchling Plasma	0.5	40	4	IgM	10,000
			40	4	IgG	10,000
	Day 8 Plasma	1	20	2	IgM	5,000
			20	2	IgG	5,000
PC-OVA	Hen Plasma	1	40	4	IgM	20,000
			40	4	IgG	20,000
	Yolk	1	40	4	IgM	5,000
			40	4	IgG	5,000
	Albumen	1	10	2	IgM	5,000
			10	2	IgG	5,000
	Hatchling Plasma	1	40	4	IgM	20,000
			40	4	IgG	20,000
	Day 8 Plasma	1	40	4	IgM	5,000
			40	4	IgG	10,000
OVA	Hen Plasma	1	40	4	IgM	20,000
			40	4	IgG	20,000
	Yolk	1	40	4	IgM	10,000
			40	4	IgG	10,000
	Albumen	1	10	2	IgM	10,000
			10	2	IgG	10,000
	Hatchling Plasma	1	40	4	IgM	20,000
			40	4	IgG	20,000
	Day 8 Plasma	1	20	2	IgM	5,000
			10	2	IgG	10,000