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. 2019 May 20;2:189. doi: 10.1038/s42003-019-0435-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Characterization of free-floating and total miR-21 in healthy human plasma using the integrated microfluidic platform and benchmarked against conventional protocol using RT-qPCR. a The voltage shift ΔV for each analysis both before (free-floating) and after (total) SAW-induced EV lysis showing consistent results across the three donors. b Free-floating and total miR-21 expression level in healthy human plasma using both the integrated microfluidic platform and RT-qPCR protocol. For the expression level, the mean free-floating value measured by both the integrated device and RT-qPCR has been set to 1.0, and lysed exosome measurements are referenced to these values, respectively. Two different donor cohorts, each consisting of three healthy human plasma samples, were used for the microfluidic platform (donors 1–3) and RT-qPCR protocol (donors 4–6), respectively. For both panels, the mean reflects the average of all nine data points, and the error bars represent the uncertainty at 95% confidence