Figure 4.
Vtd reduction enhances notching phenotypes in mts knockdown wing. Effects of RNAi knockdown of mts and/or vtd driven by vg-Gal4 in the wing. (a–d) Female adult wings of indicated genotypes. Double knockdown of mts and vtd shows severe wing reduction (d). Scale bar, 100 µm. (e–h) Cut staining in wing imaginal disc from 3rd instar larvae. Cut staining is strongly reduced or lost at the DV boundary of wing imaginal disc (white arrows). Scale bar, 50 µm. (i–l) GFP expression by vg-Gal4 show the presence of DV boundary cells. (m–p) Merges of (e–l). (g–t) Female adult wings of indicated genotypes. mts knockdown in vtd heterozygote (t) enhances the notching phenotype by mts RNAi (r). (u–x) Cut staining of wing imaginal discs of the genotypes shown in (q–t). Scale bar, 50 µm. (y,z) Quantification of wing phenotypes. White: normal wing, Light grey: mild wing notching (less than 40% reduction in wing size), Dark grey: severe wing notching (41–70% reduction in wing size), Black: near complete wing loss (more than 71% reduction in wing size) (y) Quantification of adult wing phenotypes in (a–d). (z) Quantification of adult wing phenotypes in (q–t).