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. 2019 May 20;9:7615. doi: 10.1038/s41598-019-44151-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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FLIM-FRET analysis shows heterogeneous aggregation of mutant and wild type HTT. Exemple of EGFP-HTT-74Q (a) and RFP-HTT-20Q (b) signal in HeLa cells. (c) Average EGFP lifetime obtained fitting single-pixel distribution with four exponents. Decay time in pixel with less than 150 counts was set to zero. (d) The decay signal distributions from two regions (30 × 30 pixels) show different exponential tails, in agreement with average lifetime estimates. (e) Average lifetimes calculated from lifetime distribution over regions of 30 × 30 pixels in 11 cells positive to EGFP-HTT-74Q and 14 cells positive to both EGFP-HTT-74Q and RFP-HTT-20Q transfection.