Fig. 3.

Higher Ras/Pten-induced tumor incidence at the region adjacent to the squamocolumnar junction (SCJ). a, b Experimental scheme. c Macroscopic phenotype indicates significant tumor formation throughout the region adjacent to the SCJ. d Tumor incidence from tumor-prone Keratin15⁺ (Krt15⁺) progenitors. Control, n = 7 animals. Krt15-CrePR; LSL-Kras^G12D; Pten^wt/flox, n = 8 animals. Statistical significance was determined by Fisher’s exact test; ns = not significant, **p < 0.005. e Histology and tdTomato lineage tracing revealed tumors from tumor-prone Krt15⁺ progenitors adjacent to the SCJ, while distant progenitors do not show aberrant growth. Scale bars, 100 µm