Fig. 3.
Pre-Tfh cells among lymphoid cells. A–F Cells were gated according to forward scatter/side scatter (FSC/SSC) (A) and then by PI and CFSE (B). Only PI-negative and CFSE-positive cells (i.e., live cells) were gated and then analyzed for pre-Tfh cells, including CD4+CXCR5+ (C, D) and CD4+ICOS+ T cells (E, F). (G) Percentage of CD4+ICOS+ T cells in each group (mean ± SD; data from three independent experiments with 6 rats per condition per experiment; **P < 0.01).