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. 2019 Feb 22;35(3):507–518. doi: 10.1007/s12264-019-00344-1

Fig. 3.

Fig. 3

Pre-Tfh cells among lymphoid cells. AF Cells were gated according to forward scatter/side scatter (FSC/SSC) (A) and then by PI and CFSE (B). Only PI-negative and CFSE-positive cells (i.e., live cells) were gated and then analyzed for pre-Tfh cells, including CD4+CXCR5+ (C, D) and CD4+ICOS+ T cells (E, F). (G) Percentage of CD4+ICOS+ T cells in each group (mean ± SD; data from three independent experiments with 6 rats per condition per experiment; **P < 0.01).