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. 2019 Feb 21;35(3):497–506. doi: 10.1007/s12264-019-00347-y

Fig. 2.

Fig. 2

Validation of shRNA efficiency and specificity in neuroligin knockdown in HEK293T cells. A–C Immunoblots of NLs after shRNA knockdown in HEK293T cells. A shNL1 specifically and efficiently knocked down NL1 expression (0.082 ± 0.033 for shNL1, 1.436 ± 0.204 for shNL1-scr, 0.705 ± 0.171 for shNL1-2, 1.013 ± 0.070 for shNL2, and 1.019 ± 0.059 for shNL3, normalized to control). B shNL2 specifically and efficiently knocked down NL2 expression (1.594 ± 0.235 for shNL1, 1.337 ± 0.169 for shNL1-scr, 1.135 ± 0.049 for shNL1-2, 0.110 ± 0.033 for shNL2, and 1.430 ± 0.068 for shNL3, normalized to control). C shNL3 specifically and efficiently knocked down NL3 expression (0.783 ± 0.323 for shNL1, 1.293 ± 0.063 for shNL1-scr, 1.153 ± 0.215 for shNL1-2, 0.897 ± 0.085 for shNL2, and 0.166 ± 0.078 for shNL3, normalized to control; one-way AVONA, *P <0.05, **P <0.01, ***P <0.001; mean ± SEM; n = 3 independent experiments).