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. 2019 Feb 21;35(3):497–506. doi: 10.1007/s12264-019-00347-y

Table 1.

Sequences of primer pairs.

shRNA Primer Sequence
shNL1

Forward: GATCTCCGGAAGGTACTGGAAATCTATTCAAGAGATAGATTTCCAGTACCTTCCTTTTTGGAAC

Reverse: TCGAGTTCCAAAAAGGAAGGTACTGGAAATCTATCTCTTGAATAGATTTCCAGTACCTTCCGGA
shNL1 scrambled

Forward: GATCTCCGAGTATTATCAGGACGGAATTCAAGAGATTCCGTCCTGATAATACTCTTTTTGGAAC

Reverse: TCGAGTTCCAAAAAGAGTATTATCAGGACGGAATCTCTTGAATTCCGTCCTGATAATACTCGGA
shNL1-2

Forward: GATCTCCGGTTTCCTCATATGTGCAATTCAAGAGATTGCACATATGAGGAAACCTTTTTGGAAC

Reverse: TCGAGTTCCAAAAAGGTTTCCTCATATGTGCAATCTCTTGAATTGCACATATGAGGAAACCGGA
shNL2

Forward: GATCTCCGGAGCAAGTTCAACAGCAATTCAAGAGATTGCTGTTGAACTTGCTCCTTTTTGGAAC

Reverse: TCGAGTTCCAAAAAGGAGCAAGTTCAACAGCAATCTCTTGAATTGCTGTTGAACTTGCTCCGGA
shNL3

Forward: GATCTCCGTAGCCTGGTCCAAATACATTCAAGAGATGTATTTGGACCAGGCTACTTTTTGGAAC

Reverse: TCGAGTTCCAAAAAGTAGCCTGGTCCAAATACATCTCTTGAATGTATTTGGACCAGGCTACGGA