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. 2019 Feb 27;24(3):539–547. doi: 10.1007/s12192-019-00983-3

Table 1.

Primers used to amplify heat shock proteins transcripts of interest by qRT-PCR. Forward (Fwd) and reverse (Rev) primers

Hsp60

Fwd

Rev

5′- GTCACCATGGGTCCAAAGGGC -3′

5′- TTCTTTGGCAATAGAACGGGC -3′

98.7% 61.0 °C
Hsp70

Fwd

Rev

5′- GACGAGAAGCAAAGGCAAAG -3′

5′- CCAATGAGTTGCTGTCTAACCA -3′

108.9% 58.6 °C
Hsp90

Fwd

Rev

5′- GACCTTGTAAACAACTTGGGT -3′

5′- ACACCAAACTGTACCAATCAT -3’

101.9% 64.0 °C
Hsp Beta-1

Fwd

Rev

5′- GAAGAGATTGTAGTTAAAACC -3′

5′- TTAGTGTTGAATTGGAATAAG -3′

95.9% 61.0 °C
α-tubulin

Fwd

Rev

5′- GARTTCCARACCAACTTGGT -3′

5′- GCCATGTAWTTGCCGTGSCG -3′

104.4% 55.0 °C