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. 2019 May 20;9:7587. doi: 10.1038/s41598-019-44123-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Changes in the expression of intercellular junctions during the senescence of PMCs in vitro. The results of the quantitative examination of SA-β-Gal, connexin 43, E-cadherin, occludin, and desmoglein in young (Young) and senescent (Sen) PMCs. (a) Representative pictures showing the decreased expression of junctional proteins in the senescent PMCs. (b) Experiments were performed with 8 cultures of PMCs obtained from different donors. The results are expressed as the mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001 vs. Young. RFU – Relative Fluorescence Units (magnification ×200).