Figure 7.

Inhibition of the release of tritium into the medium following incubation of T84 cells with 100 nM [Ara-³H]AEA in the presence of 1 µM URB597. In Panel A, cells or wells alone were preincubated for 10 min with 1 µM URB597 followed by incubation for 10 min with 100 nM [Ara-³H]AEA. The samples were then washed three times and then incubated in medium containing URB597 ± CBX or Mef and samples of the medium were taken over the next ten minutes. For Panel A, the data are means ± SD, N = 8. The radiolabel in medium is calculated taking into account the amount already released and the volume of medium remaining (see Methods). The inset in Panels A shows the data for the first 2 minutes. In Panel B, the individual areas under the curve for 10 min period studied (AUC_0–10) are shown. Data for each experiment and “source” (cell or well) are connected by the lines (N = 8). A two-way randomised block ANOVAe with Geisser-Greenhouse-adjusted degrees of freedom gave P < 0.001 for test compound, source and for the interaction test compound x source. In view of the significant interaction, one-way randomised block ANOVAe with Geisser-Greenhouse-adjusted degrees of freedom were calculated for the cells and wells separately. Both showed significant P values (P ≤ 0.0006) and significant matching of the samples (P ≤ 0.0002). **P < 0.01, ***P < 0.001 vs URB alone, Dunnett’s multiple comparisons test.