Fig. 1.

Schematic illustration of BCW synthesis. a Sequential HCR and PC on the live cell. HCR: hybridization chain reaction. PC: polyelectrolyte complexation. b Formation of the supramolecular DNA template and the concomitant DNA-templated alginate assembly during HCR. Cholesterol-conjugated DNA initiators (i.e., cholesterol-DIs) are inserted into the plasma membrane through cholesterol-lipid interactions. Cells with DNA initiators are incubated in the solution of DM1 and alginate-DM2 for the formation of the template on the plasma membrane. The arrows indicate multiple repeating units. c DNA-templated crosslinking of alginate and polylysine via PC