Fig. 1.

EGFR was localized more in the early vesicular compartment of enterocytes from patients with CD respect to controls. a Images of immunofluorescence staining for EGFR (magenta), EEA1 (green), and nuclei (blue) in crypts and villi of intestinal biopsies from patients with CD (GCD, gluten-containing diet; GFD, gluten-free diet) and controls (CTR). The white color in the merge panels indicates co-localization of EGFR and EEA1. Representative fields. Scale bar = 20 µm. b Statistical analysis of EGFR fluorescence intensity in selected epithelial areas. c Statistical analysis of the co-localization coefficients of EGFR with EEA1 staining. d Statistical analysis of fluorescence intensity of EEA1 staining in selected areas of crypt and villi enterocytes. One-way ANOVA Bonferroni corrected. *p < 0.05, **p < 0.01, ***p < 0.001