Fig. 8.

Treatment with P31-43 induced in skin fibroblasts a transient delay of the EGF/EGFR trafficking in controls and a prolonged delay in CD patients. EGF/EGFR trafficking after P31-43 in control and CD fibroblasts. a Images of immunofluorescence staining for EGFR and EEA1 in skin fibroblasts from patients and controls treated with P31-43 for 10′ (T0) and then with both EGF and P31-43 for 30 min, 1, 3, 6, or 24 h. The white color in the merge panels indicates co-localization of EGFR (magenta) and EEA1 (green), nuclei are in blue. Representative fields. Scale bar = 20 µm. b Statistical analysis of the co-localization coefficient of EGFR with EEA1. In all experiments, samples from five subjects from each group of patients (GCD CD and GFD CD) and controls (CTR) were examined. Three independent experiments were performed using samples from each group. Columns represent the means, and bars represent standard deviations; hatched columns indicate P31-43 treatment. One-way ANOVA Bonferroni corrected. *p < 0.05, **p < 0.01, ***p < 0.001