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. 2019 May 20;9:7617. doi: 10.1038/s41598-019-44098-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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FAK/Pyk2 activity promotes IL-1β-mediated pro-inflammatory molecule expression via transcriptional control. (a) HAoECs were treated with DMSO or PF-271 (2.5 μM) for 1 h prior to IL-1β (20 ng/ml) stimulation for 0 to 60 min. Cropped images of immunoblotting for pY397 FAK, FAK, active NF-κB (pS536 NF-κB), NF-κB, IκBα, active ERK (p-ERK), ERK, active JNK (p-JNK1, 2, and 3), JNK, active p38 (p-p38), p38, and β-actin as loading control are shown. Full length blots shown in Supplemental Fig. 13. (b) HAoECs were treated with DMSO or PF-271 (2.5 μM) for 1 h prior to IL-1β (20 ng/ml, 6 h) stimulation. RNA was collected, and RT-qPCR for cell adhesion molecules was performed (n = 3, ±SEM). (c) HAoECs were treated with DMSO or PF-271 (2.5 μM) for 1 h prior to IL-1β (20 ng/ml, 24 h) stimulation. RNA was collected, and RT-qPCR using an array for inflammatory cytokines, chemokines and their receptors. Shown are a selection of genes that are important in vascular inflammation and were reduced by PF-271 treatment upon IL-1β stimulation. (d) CXCL11, MCP-1, and IP-10 mRNA levels were verified by RT-qPCR (n = 3, ±SEM). **p < 0.01, ***p < 0.001, ****p < 0.0001.