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. 2019 May 20;9:7602. doi: 10.1038/s41598-019-44086-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Southern blot confirms the targeted deletion of CPCT. Genomic DNA samples from WT, CPCT+/−, and cpct⁻ parasites were digested with restriction enzyme SacII and separated on agarose gels. Blots were probed with radiolabeled DNA fragments recognizing the ORF (A) or an upstream flanking region of CPCT. (B) The replacement of CPCT alleles by PAC and HYG is indicated. Full-size, unedited blots and loading controls are presented in Supplementary Fig. S1.