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. 2019 May 20;9:7602. doi: 10.1038/s41598-019-44086-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Endoplasmic Reticulum (ER) localization of CPCT in L. major. Log phase promastigotes of cpct⁻/+GFP-CPCT were labeled with rabbit anti-T. brucei BiP antiserum followed by a goat anti-rabbit IgG-Texas Red antibody and subjected to confocal immunofluorescence microscopy. (A) Phase contrast; (B) GFP fluorescence; (C) Anti-BiP staining; (D) Merge of B and C. Scale bar: 5 µm. The overlap between BiP and GFP-CPCT was determined by the JaCOP Image J analysis of 30 cells (Table S1).