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. 2019 May 20;9:7602. doi: 10.1038/s41598-019-44086-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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CPCT is required for incorporating choline to PC. (A) Lane 1: [¹⁴C]-Choline-P. Lane 2: [¹⁴C]-CDP-choline. Lane 3–5: cell lysates from E. coli transformed with pET-ScCPCT, pET-LmCPCT, or pET only were incubated with [¹⁴C]-labeled choline-P, followed by TLC analysis as described in METHODS. (B,C) Log phase Leishmania promastigotes were cultivated in the presence of [³H]-labeled choline (B) or [³H]-labeled EtN (C). Total lipids were extracted after 48 hours and analyzed by TLC. O: origin of loading; LPC: lyso-phosphatidylcholine; LPE: lyso-phosphatidylethanolamine. Full-size, unedited images are presented in Supplementary Fig. S4.