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. 2019 May 20;9:7602. doi: 10.1038/s41598-019-44086-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cpct⁻ mutants contain normal levels of PC. Total lipids from log phase (A–C) and stationary phase (D–F) promastigotes were examined by ESI/MS in the positive ion mode. (A,D): WT; (B,E): cpct⁻; (C,F): cpct⁻/+CPCT. Peaks corresponding to LPC and diacyl-PC are indicated. Std: PC standard (14:0/14:0-PC) for quantitation. (G) Abundance of PC (diacyl-PC + LPC) and PE in L. major promastigotes were determined and averaged from three independent experiments. Error bars represent standard deviations from three biological repeats.