Figure 8.

Cpct⁻ mutants show significant growth delay when co-cultured with bacteria and the growth delay can be rescued by EtN supplementation. Effects of Serratia marcescens and Enterobacter cloacae on the growth of cpct⁻ mutants were determined in a co-culture experiment. L. major promastigotes (1 × 10⁶ cells/ml) were incubated in lipid-free M199 medium alone or with Serratia marcescens (50 bacteria/ml) or Enterobacter cloacae (50 bacteria/ml). EtN (250 μM) was included as indicated. The number of promastigotes/ml was recorded using a hemocytometer after 24 h. Results were averaged from three independent experiments with triplicates. Error bars represent standard deviations from three biological repeats (*p < 0.05; ***p < 0.001) based on one way Anova relative to WT.