Fig. 3.
Surplus of APP or BACE1 differentially affect APP processing. Using SH-SY5Y cells stably expressing APP695 or BACE1, cleavage of APP was analyzed by western blot and ultra-sensitive MSD assay. Representative western blots for the examination of APP, BACE1, sAPPβ, and sAPPtotal (a, b), and the corresponding quantification for the relative amounts of sAPPβ (c) and sAPPtotal (d). MSD multiplexing to quantify the absolute amounts of Aβ34 (e), Aβ38 (f), Aβ40 (g), and Aβ42 (h). Data were collected from four independent experiments. Bars and error bars indicate mean ± s.e.m. c–h Data were analyzed with one-way ANOVAs and Tukey’s post-hoc tests were performed for pairwise comparisons; selected comparisons are highlighted ***p < 0.001, *p < 0.05, ns = nonsignificant p > 0.05. c sAPPβ, F(2, 9) = 131.2, p < 0.0001, d sAPPtotal, F(2, 9) = 190.3, p < 0.0001, e Aβ34, F(2, 9) = 70.04, p < 0.0001, f Aβ38, F(2, 9) = 149.0, p < 0.0001, g Aβ40, F(2, 9) = 89.96, p < 0.0001, h Aβ42, F(2, 9) = 113.6, p < 0.0001