Optogenetic inhibition of glutamatergic neurons in the POR impairs spatial memory. A Schematic of novel object place recognition test. B, C Animal groups and laser illumination protocol used during novel object place recognition. On: laser on, off: laser off, G1: Group 1. D, F Object exploration data in the study phase to objects 1 and 2 (P < 0.05 by paired t-test). E, G Comparison of D2 of mCherry+ group and hChR2+ group or eNpHR3.0+ group (n1: mCherry+ group, n2: hChR2+ group or eNpHR3.0+ group, *P < 0.05, Student’s t-test). Data are presented as mean ± SEM.