Fig. 2.

The siRNA molecules interfere with mRNA expression of KLF5 in the chicken SMSCs. a Interference efficiency among the three different KLF5-siRNA on 24 h. b Expression of KLF5 at 0, 24, 48, and 72 h after transfection of siRNA1 and negative siRNA. The mRNA expression level was measured by qPCR, normalized to β-actin and measured with 2^(−ΔΔCt) value. Data are expressed as the mean ± SEM. n = 3. **p < 0.01 vs the negative siRNA groups