Figure 5.

Bleeding from the tail and ex vivo PT and aPTT measurements of BALB/c mice during subcutaneous infection. Male BALB/c mice were subcutaneously infected with 5 × 10⁴ culture-derived T. cruzi trypomastigotes (Tulahuén strain). (A) Bleeding was caused by a tail transection in NI, 7 and 14 dpi SC mice. Absorbance at 540 nm (hemoglobin concentration) was used to estimate blood loss. (B,C) NI or SC plasma samples were obtained by cardiac puncture followed by addition of aPTT or PT reagent. Clotting time was estimated using a coagulometer. (A-C) Values are presented as mean ± SEM for each group/day post-infection and are representative of one experiment. Results were analyzed using one way ANOVA with Tukey's multiple comparisons test (*0.0001 < p < 0.05).