Table 1.
Summary of assays performed to study the role of T1F in Salmonella adhesion and/or invasion of cell lines.
| No. | Serovar | Strains | Bacteria- growth conditions | Cell line and growth conditions | Outcome | Source |
|---|---|---|---|---|---|---|
| 1. | Typhimurium | TML, W118, NY, PR (T1F+); S850, S2204 (T1F-) | Temp: ? Time: ? Medium: ? O2: ? Shaking: ? |
HeLa Medium: ? Incubation : 30 min or 3 h |
MR (non-T1F-mediated) adhesion to HeLa cells was observed | Jones and Richardson, 1981 |
| 2. | Typhimurium | S6354, S6358, S1566, S850F (T1F+); S6351, S6352, S1566, S2204, S8x‘50 (T1F-) | Temp: 37°C Time: 4 SP, 48h Medium: tubes of 10 ml nutrient broth O2: aerobically Shaking: No |
HEp-2, HeLa Medium: MEM + 0.5% FBS Incubation: 30 min or 90 min |
MS adhesion of T1F+ strains | Tavendale et al., 1983 |
| 3. | Typhimurium | 6354, 6358 (T1F+) | Temp: 37°C Time: 4 SP, 48h Medium: tubes of 10 ml nutrient broth O2: aerobically Shaking: No |
HEp-2 Medium: MEM + 0.5% FBS Incubation: 30 min |
MS adhesion | Old et al., 1986 |
| 4. | Typhimurium | SR-11 X3306 | Temp: 37°C Time: grown till logarithmic growth phase Medium: brain heart infusion broth O2: Anaerobic growth in an atmosphere of 5% C02,10% H2, and 85% N2 (T1F+); aerobic growth was carried out in a gyratory shaker (T1F-) Shaking: ? |
HEp-2 Medium: MEM Incubation: 3 h |
MS adhesion of T1F+ strain | Ernst et al., 1990 |
| 5. | Typhimurium, Braenderup | Typhimurium- 501 (T1F+); 501NP, 503 (T1F-);Braenderup- 301, 302 (T1F+); 302NP, 303 (T1F-) | Temp: ? Time: overnight Medium: L-broth or L-agar plate O2: ? Shaking: ? |
HeLa Medium: MEM Incubation: 2 h or 18 h |
MS adhesion of T1F+ strain | Horiuchi et al., 1992 |
| 6. | Typhimurium | IR715, a derivative of ATCC 14028; SR-11 derivativeAJB3 (T1F+); AJB4 (Δfim, T1F-) | Temp: ? Time: ? Medium: broth O2: aerobically Shaking: No |
HEp-2, T-84, Int-407, HeLa, MDCK Medium: ? Incubation: ? |
T1F-dependent adhesion to HeLa | Bäumler et al., 1996 |
| 7. | Enteritidis | S1400/94; LA5, 27655R | Temp: 37°C Time: 24h Medium: nutrient broth O2: aerobically Shaking: No |
INT-407, Caco-2 Medium: EMEM+10% FBS Incubation: 2 h |
T1F-dependent adhesion, Int-407- MS adhesion of T1F+ strain | Dibb-Fuller et al., 1999 |
| 8. | Typhimurium | SR-11 derivates- X 4252 (T1F+), 4253 (T1F-) | Temp: 37°C Time: untilbacteria were in mid-log phase growth Medium: LB O2: ? Shaking: Static |
SI-H10, MM45T.BL Medium: ? Incubation: 1 h |
T1F-dependent adhesion to SI-H10 | Thankavel et al., 1999 |
| 9. | Enteritidis | - | Temp: 37°C Time: 48 to 72 h Medium: CFA or T O2: aerobically Shaking: No |
HT-29, Caco-2 Medium: ? Incubation: ? |
HT-29- T1F- strain invades better, Caco-2- T1F- strain invades equally, | Rajashekara et al., 2000 |
| Temp: 37°C Time: 24 h Medium: CFAbroth O2: aerobically Shaking: Yes (gentle shaking) |
HD-11, MQ-NCSU Medium: Hank’s balanced salt solution Incubation: 45 min |
No differences | ||||
| 10. | Gallinarum; Pullorum; Typhimurium | 297; 2933; LT2 | Temp: 37°C Time: 48h Medium: Luria broth O2: aerobically Shaking: No |
HEp-2 Medium: ? Incubation: ? |
Presence of Typhimurium T1F increases adhesion and invasion rate of Gallinarum and Pullorum | Wilson et al., 2000 |
| 12. | Typhimurium, Enteritidis | Typhimurium- no information, Enteritidis- isolate no. 327 | Temp: 37°C Time: 5 passages Medium: LB broth O2: ? Shaking: No |
HT-29 Medium: a-MEM+10% FBS; Hu 1703He Medium: Fib41B+10% FBS; Incubation: 2 h |
MS adhesion to cell lines | Kisiela et al., 2006 |
| 13. | Typhimurium | BJ2710- SL1344 derivative containing the LB5010 fimH gene (T1F+);BJ2508- BJ2710 fimH::kan (T1F-) | Temp: 37°C Time: 48 h Medium: 10 ml of LB broth O2: ? Shaking: No |
HEp-2 Medium: RPMI+10% FBS Incubation: 24 hchicken intestinal epithelium Medium: RPMI+7% FBS+3%chicken serum Incubation: 24 h |
T1F-dependent biofilm formation | Ledeboer et al., 2006 |
| 14. | Typhimurium | SL1344, ALB3, LB5010,Isogenic model with expression of FimH variants | Temp: 37°C Time: overnight Medium: SB O2: ? Shaking: No |
HEp-2 Medium: ? Incubation: 1 h |
FimH variant dependent adhesion | Kisiela et al., 2011 |
| 15. | Typhimurium | SL1344 | Temp: ? Time: ? Medium: ? O2: ? Shaking: ? |
HeLa Medium: DMEM+10% FBS Incubation: 10′, 12′ |
T1F-dependent adhesion, MS adhesion | Misselwitz et al., 2011 |
| 16. | Gallinarum | Gallinarum- isolate no. 589/02 (1); ΔfimH mutant (2); Gallinarum with Enteritidis FimH variant (3) | Temp: 37°C Time: passaged five times Medium: LB broth O2: ? Shaking: No |
HT-29 Medium: ? Incubation: 2 h |
1- No adhesion 2- MR adhesion 3- MS adhesion |
Kuźmińska-Bajor et al., 2012 |
| 17. | 22 serovars from subspecies I, 11 isolates from subspecies II-VII | Isogenic model with expression of FimH variants | Temp: ? Time: overnight Medium: ? O2: ? Shaking: ? |
HEp-2, RAW264.7 Medium: ? Incubation: 1 h; |
FimH variant dependent adhesion, MS adhesion | Kisiela et al., 2012 |
| 18. | Enteritidis | Isolate no. 327 | Temp: 37°C Time: passaged five times Medium: LB broth O2: ? Shaking: No |
ICE-1 Medium: IEC medium Incubation: 2 h |
FimH dependent adhesion and invasion, MS adhesion and invasion | Kuźmińska-Bajor et al., 2015 |
| 19. | Choleraesuis | Isogenic model in Choleraesuis with expression of FimH variants | Temp: 37°C Time: passaged five times Medium: LB broth O2: ? Shaking: No |
IPEC-J2 Medium: DMEM+10% FBS Incubation: 2 h |
FimH variant dependent adhesion and invasion | Grzymajlo et al., 2017 |
| 20. | Typhimurium, Enteritidis, Gallinarum, Choleraesuis, Dublin | Isogenic model in Typhimurium with expression of FimH variants | Temp: 37°C Time: 48 h Medium: LB broth O2: ? Shaking: No |
HEp-2,IPEC-J2 Medium: D-MEM/Ham’s F12 + 5% Incubation: 2 h |
HEp-2- FimH variant dependent adhesion and invasionIPEC-J2- not-FimH- dependent adhesion and invasion | Kolenda et al., 2018 |
Temp., Temperature; O2, presence of oxygen during growth; MR, mannose resistant; MS, mannose sensitive; T1F, type 1 fimbriae; “T1F+”, type 1 fimbriae expressing; “T1F-”, type 1 fimbriae non-expressing.