Antibodies |
|
Rabbit polyclonal anti-Sth1 |
Gift from Cairns Lab |
N/A |
Mouse monoclonal anti-Scc1 (362 D11B10) (budding yeast) |
Gift from Shirahige Lab |
N/A |
Mouse monoclonal anti- α-tubulin (TAT-1) |
Cell Services Science Technology Platform, The Francis Crick Institute |
N/A |
Mouse monoclonal anti-V5 |
Bio-Rad |
Cat# MCA1360 |
Mouse monoclonal anti-HA (F7) |
Santa Cruz |
Cat# sc-7392 |
Mouse monoclonal anti-HA (12CA5) |
Cell Services, The Francis Crick Institute |
N/A |
Rabbit Peroxidase Anti-Peroxidase |
Sigma-Aldrich |
Cat# P1291 |
Anti-mouse IgG (HRP-conjugated) |
GE Healthcare |
Cat# NA931 |
Anti-rabbit IgG (HRP-conjugated) |
GE Healthcare |
Cat# NA934 |
Anti-AID Tag (IAA17) Protein |
2B Scientific |
Cat# CAC-APC004AM |
|
Chemicals, Peptides, and Recombinant Proteins |
|
α-factor |
Peptide Chemistry Science Technology Platform, The Francis Crick institute |
N/A |
Nocodazole |
Sigma-Aldrich |
Cat# M1404 |
Indole-3-acetic acid (IAA) |
Sigma-Aldrich |
Cat# I3750 |
G418 |
Sigma-Aldrich |
Cat# G8618 |
Hygromycin B |
Invitrogen |
Cat# 10687010 |
Formaldehyde solution |
Sigma-Aldrich |
Cat# 252549 |
Phenylmethylsulfonyl fluoride (PMSF) |
Sigma-Aldrich |
Cat# P7626 |
Pefabloc SC |
Roche |
Cat# 11 429 876 001 |
cOmplete EDTA-Free Protease Inhibitor Cocktail |
Sigma-Aldrich |
Cat# 04693132001 |
Zymoliase 100T |
MP |
Cat# 320931 |
Micrococcal Nuclease |
ThermoFisher |
Cat# EN0181 |
Proteinase K |
ThermoFisher |
Cat# EO0491 |
Benzonase Nuclease |
Sigma-Aldrich |
Cat# E1014 |
RNase A |
Sigma-Aldrich |
Cat# 10109169001 |
Protein Assay Dye |
Bio-Rad |
Cat# 5000006 |
Propidium iodide solution |
Sigma-Aldrich |
Cat# P4864 |
InstantBlue Coomassie Protein Stain |
Expedeon |
Cat# HG773010
|
GelRed Nucleic Acid Gel Stain |
Biotium |
Cat# 41003-1 |
PowerUp SYBR Green Master Mix |
ThermoFisher |
Cat# A25742 |
AcTEV Protease |
ThermoFisher |
Cat# 12575015 |
PreScission Protease |
GE Healthcare |
Cat# 27084301 |
ATP |
Sigma-Aldrich |
Cat# A2383 |
Phosphocreatine di(tris) salt |
Sigma-Aldrich |
Cat# P1937 |
Creatine Kinase |
Sigma-Aldrich |
Cat# 10127566001 |
PstI |
New England Biolabs |
Cat# R0140S |
Isopropyl β-D-1-thiogalactopyranoside (IPTG) |
Sigma-Aldrich |
Cat# 10724815001 |
Flag peptide |
Peptide Chemistry Science Technology Platform, The Francis Crick institute |
N/A |
Budding yeast cohesin (Smc1-Smc3-Scc1-Scc3) |
Minamino et al., 2018 |
N/A |
Budding yeast Scc2-Scc4 |
Minamino et al., 2018 |
N/A |
Budding yeast Scc2C |
Minamino et al., 2018 |
N/A |
Budding yeast Histones |
Kurat et al., 2017 |
N/A |
Budding yeast Nap1 |
Kurat et al., 2017 |
N/A |
Budding yeast ISW1A |
Kurat et al., 2017 |
N/A |
Budding yeast RSC |
Wittmeyer et al., 2004 |
N/A |
|
Critical Commercial Assays |
|
InFusion HD cloning kit |
Clontech Laboratories |
Cat# 639634 |
CloneAmp HiFi PCR Premix |
Clontech Laboratories |
Cat# 639298 |
Q5 Site-Directed Mutagenesis Kit |
New England Biolabs |
Cat# E05545
|
Dynabeads Protein A |
ThermoFisher |
Cat# 10002D |
Dynabeads M-270 Epoxy |
ThermoFisher |
Cat# 14302D |
Rabbit immunoglobulin G (IgG) |
Sigma-Aldrich |
Cat# I5006 |
ECL Prime Western Blotting Detection Regent |
GE Healthcare |
Cat# RPN2232 |
Human IgG-Agarose |
Sigma-Aldrich |
Cat# A6284-5ML |
Calmodulin Affinity Resin |
Agilent Technologies |
Cat# 214303 |
HiTrap Heparin HP 1 ml |
GE Healthcare |
Cat# 17040601 |
Superdex 200 Increase 10/300 GL |
GE Healthcare |
Cat# 28990944 |
Superose 6, 10/300 GL |
GE Healthcare |
Cat# 17517201 |
HiTrap Heparin HP 5 ml |
GE Healthcare |
Cat# 17040701 |
HiLoad 16/600 Superdex 200 |
GE Healthcare |
Cat# 28-9893-35 |
Glutathione Sepharose 4B |
GE Healthcare |
Cat# 17075601 |
Mono Q 5/50 GL, 1 ml |
GE Healthcare |
Cat# 17516601 |
ANTI-FLAG M2 Affinity Gel |
Sigma-Aldrich |
Cat# A2220 |
Slide-A-Lyzer Dialysis Cassettes, 3.5K MWCO, 12 mL |
ThermoFisher |
Cat# 66110 |
MicroSpin S-400 HR columns |
GE Healthcare |
Cat# 27514001 |
Amicon Ultra-4 centrifuge filter unit, 10 NMWL |
MERCK MILLIPORE |
Cat# UFC801024 |
Vivaspin 20 centrifugal concentrator, 100,000 MWCO |
Sartorius |
Cat# VS2042 |
|
Experimental Models: Organisms/Strains |
|
All Saccharomyces cerevisiae strains used in this study are listed in Table S1
|
Lab stock and this study |
N/A |
Escherichia coli DH5α competent cells |
New England Biolabs |
Cat# C2987U |
|
Oligonucleotides |
|
All oligonucleotides used for qPCR are listed in Table S2. |
N/A |
N/A |
|
Recombinant DNA |
|
All plasmid DNA used in this study are listed in Table S3. |
N/A |
N/A |
|
Software and Algorithms |
|
Snapgene v2.6 |
GSL Biotech |
N/A |
FlowJo v10.1 |
FlowJo |
N/A |
ImageQuant TL v8.1 |
GE Healthcare |
N/A |
ImageJ v1.50c |
NIH, USA |
N/A |
|
Deposited Data |
|
MNase sequencing data |
This study |
GEO: GSE117881
|
Unprocessed gel images presented in this manuscript |
This study |
https://doi.org/10.17632/34fry4t69p.1 |