REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit polyclonal anti-Sth1	Gift from Cairns Lab	N/A
Mouse monoclonal anti-Scc1 (362 D11B10) (budding yeast)	Gift from Shirahige Lab	N/A
Mouse monoclonal anti- α-tubulin (TAT-1)	Cell Services Science Technology Platform, The Francis Crick Institute	N/A
Mouse monoclonal anti-V5	Bio-Rad	Cat# MCA1360
Mouse monoclonal anti-HA (F7)	Santa Cruz	Cat# sc-7392
Mouse monoclonal anti-HA (12CA5)	Cell Services, The Francis Crick Institute	N/A
Rabbit Peroxidase Anti-Peroxidase	Sigma-Aldrich	Cat# P1291
Anti-mouse IgG (HRP-conjugated)	GE Healthcare	Cat# NA931
Anti-rabbit IgG (HRP-conjugated)	GE Healthcare	Cat# NA934
Anti-AID Tag (IAA17) Protein	2B Scientific	Cat# CAC-APC004AM
Chemicals, Peptides, and Recombinant Proteins
α-factor	Peptide Chemistry Science Technology Platform, The Francis Crick institute	N/A
Nocodazole	Sigma-Aldrich	Cat# M1404
Indole-3-acetic acid (IAA)	Sigma-Aldrich	Cat# I3750
G418	Sigma-Aldrich	Cat# G8618
Hygromycin B	Invitrogen	Cat# 10687010
Formaldehyde solution	Sigma-Aldrich	Cat# 252549
Phenylmethylsulfonyl fluoride (PMSF)	Sigma-Aldrich	Cat# P7626
Pefabloc SC	Roche	Cat# 11 429 876 001
cOmplete EDTA-Free Protease Inhibitor Cocktail	Sigma-Aldrich	Cat# 04693132001
Zymoliase 100T	MP	Cat# 320931
Micrococcal Nuclease	ThermoFisher	Cat# EN0181
Proteinase K	ThermoFisher	Cat# EO0491
Benzonase Nuclease	Sigma-Aldrich	Cat# E1014
RNase A	Sigma-Aldrich	Cat# 10109169001
Protein Assay Dye	Bio-Rad	Cat# 5000006
Propidium iodide solution	Sigma-Aldrich	Cat# P4864
InstantBlue Coomassie Protein Stain	Expedeon	Cat# HG773010
GelRed Nucleic Acid Gel Stain	Biotium	Cat# 41003-1
PowerUp SYBR Green Master Mix	ThermoFisher	Cat# A25742
AcTEV Protease	ThermoFisher	Cat# 12575015
PreScission Protease	GE Healthcare	Cat# 27084301
ATP	Sigma-Aldrich	Cat# A2383
Phosphocreatine di(tris) salt	Sigma-Aldrich	Cat# P1937
Creatine Kinase	Sigma-Aldrich	Cat# 10127566001
PstI	New England Biolabs	Cat# R0140S
Isopropyl β-D-1-thiogalactopyranoside (IPTG)	Sigma-Aldrich	Cat# 10724815001
Flag peptide	Peptide Chemistry Science Technology Platform, The Francis Crick institute	N/A
Budding yeast cohesin (Smc1-Smc3-Scc1-Scc3)	Minamino et al., 2018	N/A
Budding yeast Scc2-Scc4	Minamino et al., 2018	N/A
Budding yeast Scc2C	Minamino et al., 2018	N/A
Budding yeast Histones	Kurat et al., 2017	N/A
Budding yeast Nap1	Kurat et al., 2017	N/A
Budding yeast ISW1A	Kurat et al., 2017	N/A
Budding yeast RSC	Wittmeyer et al., 2004	N/A
Critical Commercial Assays
InFusion HD cloning kit	Clontech Laboratories	Cat# 639634
CloneAmp HiFi PCR Premix	Clontech Laboratories	Cat# 639298
Q5 Site-Directed Mutagenesis Kit	New England Biolabs	Cat# E05545
Dynabeads Protein A	ThermoFisher	Cat# 10002D
Dynabeads M-270 Epoxy	ThermoFisher	Cat# 14302D
Rabbit immunoglobulin G (IgG)	Sigma-Aldrich	Cat# I5006
ECL Prime Western Blotting Detection Regent	GE Healthcare	Cat# RPN2232
Human IgG-Agarose	Sigma-Aldrich	Cat# A6284-5ML
Calmodulin Affinity Resin	Agilent Technologies	Cat# 214303
HiTrap Heparin HP 1 ml	GE Healthcare	Cat# 17040601
Superdex 200 Increase 10/300 GL	GE Healthcare	Cat# 28990944
Superose 6, 10/300 GL	GE Healthcare	Cat# 17517201
HiTrap Heparin HP 5 ml	GE Healthcare	Cat# 17040701
HiLoad 16/600 Superdex 200	GE Healthcare	Cat# 28-9893-35
Glutathione Sepharose 4B	GE Healthcare	Cat# 17075601
Mono Q 5/50 GL, 1 ml	GE Healthcare	Cat# 17516601
ANTI-FLAG M2 Affinity Gel	Sigma-Aldrich	Cat# A2220
Slide-A-Lyzer Dialysis Cassettes, 3.5K MWCO, 12 mL	ThermoFisher	Cat# 66110
MicroSpin S-400 HR columns	GE Healthcare	Cat# 27514001
Amicon Ultra-4 centrifuge filter unit, 10 NMWL	MERCK MILLIPORE	Cat# UFC801024
Vivaspin 20 centrifugal concentrator, 100,000 MWCO	Sartorius	Cat# VS2042
Experimental Models: Organisms/Strains
All Saccharomyces cerevisiae strains used in this study are listed in Table S1	Lab stock and this study	N/A
Escherichia coli DH5α competent cells	New England Biolabs	Cat# C2987U
Oligonucleotides
All oligonucleotides used for qPCR are listed in Table S2.	N/A	N/A
Recombinant DNA
All plasmid DNA used in this study are listed in Table S3.	N/A	N/A
Software and Algorithms
Snapgene v2.6	GSL Biotech	N/A
FlowJo v10.1	FlowJo	N/A
ImageQuant TL v8.1	GE Healthcare	N/A
ImageJ v1.50c	NIH, USA	N/A
Deposited Data
MNase sequencing data	This study	GEO: GSE117881
Unprocessed gel images presented in this manuscript	This study	https://doi.org/10.17632/34fry4t69p.1