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. 2019 Jun;70(6):1072–1081. doi: 10.1016/j.jhep.2019.01.034

Fig. 3.

Fig. 3

HLA class I restriction and fine mapping of HCV-derived CD8+ T cell epitopes in donor MM. (A) Representative HLA class I restriction analysis for CD8+ T cells targeting OLP-209/210. A CD8+ T cell line specific for OLP-210 was tested for IFN-γ production after incubation with Epstein-Barr virus-immortalized B cell lines partially matched for the indicated HLA-class I alleles, either unloaded (top row) or loaded with OLP-210 (bottom row). The incorporated peptide was restricted by HLA-A*26:01. (B) Representative HLA class I restriction analysis for CD8+ T cells targeting OLP-209/210 (as in panel A). White bars: unloaded; blue bars: loaded with OLP-210. (C) Representative HLA class I restriction analysis for CD8+ T cells targeting OLP-450/451. (D, E) Fine mapping experiments for CD8+ T cell epitopes in OLP-209/210 (D) and OLP-450/451 (E). CD8+ T cell lines specific for each OLP were incubated with the indicated 8–11-mer peptides and assessed for production of IFN-γ. (F) Ex vivo staining with HLA-B*27:05/GRAAICGKY and HLA-B*27:05/GRAAICGKYLF multimers labeled with distinct fluorochromes. Left panel: donor MM (week 10 and week 32 post-infection). Right panel: control HLA-B*27:05+ donors with spontaneous resolution or treatment-induced resolution of HCV infection. HCV, hepatitis C virus; OLPs, overlapping peptides.