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. 2019 Jun;70(6):1072–1081. doi: 10.1016/j.jhep.2019.01.034

Fig. 5.

Fig. 5

The 11-mer GRAAICGKYLF epitope is not destroyed by ERAP1 allotypes expressed in donor MM. 293T E1KO cells were transfected with: (i) HLA-B*27:05; (ii) minigenes expressing the 9-mer GRAAICGKY (A), the 11-mer GRAAICGKYLF (B), or the N-terminally extended 12-mer precursor of the 9-mer epitope SRG-GRAAICGKY (C); and DNA encoding (iii) a non-functional ERAP1 mutant (E320A, left column), the PT allotype ERAP1*002 (middle column), or the ERAP1 allotype combination from donor MM (right column). Presentation of the 11-mer peptide by HLA-B*27:05 was assessed by measuring IFN-γ production after stimulation of a GRAAICGKYLF-specific CD8+ T cell line established from donor MM. (A–C) Representative dot plots. (D) Summary of 3 independent experiments ± SEM. 1-way ANOVA with Dunnett’s post hoc test was performed. **p <0.0039, *p <0.05, n.s., not significant.