Fig. 8.

Fibrin-induced reparative gene expression and cell survival signal in donor MSCs. Rat amnion-derived MSCs were cultured in fibrin glue (FG group) or standard growth medium (Control group) in vitro. n = 6 in each group, *p < 0.05. (a) Changes in cell numbers after plating 0.5 × 10⁵ MSCs were monitored at chosen time points. Repeated measures ANOVA (not significant). (b) Viability of cultured MSCs were measured at chosen time points during cultivation. Repeated measures ANOVA (not significant). (c) Gene expression of MSCs at Day 3 was assessed by real time RT-PCR. Expression in the Control group was arbitrarily defined as 1.0. Student's t-test. (d, e) Viability of MSCs (d) and LDH leakage into the medium (e) was assessed after administration of H₂O₂ to the MSC culture. Student's t-test. (f) Expression and activation of Akt and PI3K in MSCs were measured by Western blot. The proportion of the phosphorylated protein to the total protein was normalized to the Control group. Student's t-test.