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. 2019 May 14;10:559. doi: 10.3389/fphys.2019.00559

FIGURE 3.

FIGURE 3

Comparison of renal tubular apoptosis between WT and SMN+/– mice subjected to IRI. The I/R-induced AKI mouse model was generated in WT and SMN+/– mice. Kidney tissues were collected 24 h after I/R. (A) Representative images of TUNEL staining and overlays with DAPI staining. Original magnification, 400×. Bar = 50 μm. Quantitative analysis of TUNEL-positive cells; (∗p < 0.0001 vs. sham-operated WT mice; ∗∗p < 0.0001 vs. ischemic WT mice under the same experimental conditions; n = 4). (B) Representative images of immunohistochemical staining with an anti-cleaved PARP antibody. Original magnification, 400×. Bar = 50 μm. (C) Western blot analysis of apoptotic protein markers. Kidney tissue lysates were probed with anti-cleaved caspase-3 (c-caspase-3) and cleaved PARP (c-PARP) antibodies. Relative protein abundance was semi-quantified by densitometry (n = 7 or 3). Data were expressed as the mean ± SD (∗p < 0.05 vs. sham-operated WT mice, ∗∗p < 0.05 vs. ischemic WT mice under the same experimental conditions).