Table 2.
Primer and probe sequences used for the qPCR analysis of immune gene expression.
| Gene | Primers (5′–3′) | Probe (5′–3′) | Amplicon sizes (bp) | Efficiencies |
|---|---|---|---|---|
| Toll | FOR: AATGCTCGTGTTATCATGATCAAA REV: CGTGATCGTAGCCAGCGTTT |
VIC-CTGGACCACCACTAACGTCGTCGATTG-TAMRA | 76 | 93.8% |
| PPO | FOR: GCTGTGTTGCCGCAGAATG REV: AAATCCGTGGCGGTGTAGTC |
VIC-CCGCGTATCCCGATCATCATCCC-TAMRA | 67 | 97.4% |
| Lysozyme | FOR: TGTGCACAAATGCTGTTGGA REV:CGAACTTGTGACGTTTGTAGATCTTC |
VIC-ACATCACCCTAGCTTCTCAGTGCGCC-TAMRA | 76 | 96.6% |
| Relish | FOR: TCAACATAACAACACGGAGGAA REV: ATCAGGTACTAGGCAACTCATATC |
6FAM -CCCACAAATTACTTGAAGATGAACAGGACCC-TAMRA | 82 | 95.3% |
| Moricin | FOR: GGCGCAGCGATTGGTAAA REV:GGTTTGAAGAAGGAATAGACATCATG |
VIC-TCTCCGGGCGATTAACATAGCCAGC- TAMRA | 77 | 91.4% |
| EF1 | FOR: TCAAGAACATGATCACTGGAACCT REV: CCAGCGGCGACAATGAG |
6FAM -CCAGGCCGATTGCGCCGT-TAMRA | 94 | 94.0% |
| Arylphorin | FOR: CGTCAGATGCAGTCTTTAAGATCTTC REV: TGCACGAACCAGTCCAGTTC |
VIC-AATACCACGCCAATGGCTATCCGGTT-TAMRA | 112 | 96.7% |
| Armadillo | FOR: TGCACCAGCTGTCCAAGAAG REV: AAAGCGGCAACCATTTGC |
6FAM-AAGCTTCTCGCCATGCTATTATGAACTCGC-TAMRA | 70 | 92.8% |
| Tubulin | FOR: CGTGGAGCCCTACAACTCTATCC REV: GCCTCGTTGTCGACCATGA |
6FAM-ACCACCCACACCACCCTTGAGCAC-TAMRA | 81 | 100% |