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. 2019 May 21;39(5):BSR20190225. doi: 10.1042/BSR20190225

Figure 5.

Figure 5

Effect of 4-PBA on PA-induced apoptosis and oxidative stress of H9c2 cells at 24 h

(A) GRP78 expression levels in H9c2 cells after treatments. (B) CHOP expression levels in H9c2 cells after treatments. (C,D) Apoptosis analysis by flow cytometry. d1: necrotic cells; d2: late apoptotic cells; d3: live cells; d4: early apoptotic cells. (E) Caspase 3 activity in H9c2 cells after treatments. (F) BAX expression levels in H9c2 cells after treatments. (G) ROS levels in H9c2 cells after treatments. (H) NOX2 expression levels in H9c2 cells after treatments. Protein expression levels were normalized to β-actin levels. PA: 400 µM palmitic acid; 4-PBA: 500 nM 4-PBA. Data are presented as the mean ± S.E.M. of three independent experiments, *P<0.05, **P<0.01 versus the control group and &P<0.05, &&P<0.01 represent PA+4-PBA treated group versus the PA-treated group.